Autoclaved liquid medium for propagation of Treponema hyodysenteriae.
نویسندگان
چکیده
Three liquid media that differ slightly in composition but not in the method of preparation were developed for the propagation of Treponema hyodysenteriae and Treponema innocens. The three media are unique in that all components are sterilized by autoclaving before use. These media supported better growth of T. hyodysenteriae than did previously used liquid media.
منابع مشابه
Comparison of six different culture media for isolation of Treponema hyodysenteriae.
Fecal material from pigs experimentally infected with Treponema hyodysenteriae was collected and inoculated on six different selective media to compare their abilities for recovery of T. hyodysenteriae. Additionally, various types of samples were compared to find the most appropriate for submitting material to laboratories. Isolation rates for T. hyodysenteriae were 89.7% on BJ medium, 88.3% on...
متن کاملIsolation of Treponema hyodysenteriae from wild rodents.
Rodents from swine-producing farms were examined for the presence of Treponema hyodysenteriae. Wild mice (n = 257) and rats (n = 41) were trapped on eight farms. Ceca were removed aseptically, and the contents and mucosal scrapings were cultured on selective medium (blood agar containing 400 micrograms of spectinomycin per ml). T. hyodysenteriae was detected in the cecal scrapings of four mice ...
متن کاملErythrocytes as a source of essential lipids for Treponema hyodysenteriae.
Mammalian erythrocytes were tested as a nutrient source for the spirochete Treponema hyodysenteriae, the etiologic agent of swine dysentery. Brain heart infusion (BHI) broth did not support growth of T. hyodysenteriae B204 or B78. However, BHI broth supplemented with washed bovine erythrocytes, erythrocyte membranes, or cholesterol was an excellent culture medium for these strains (4 X 10(8) to...
متن کاملDetection and identification of Treponema hyodysenteriae by using oligodeoxynucleotide probes complementary to 16S rRNA.
Oligodeoxynucleotide probes (17 and 28 bases long) complementary to a unique region of Treponema hyodysenteriae 16S rRNA were developed. These probes bound specifically to partially purified rRNA and whole-cell rRNA of T. hyodysenteriae. No binding to partially purified rRNA or whole-cell rRNA of Treponema innocens, Treponema succinifaciens, Treponema bryantii, or Escherichia coli occurred unde...
متن کاملExperimental infection with Treponema hyodysenteriae in guinea pigs.
Outbred and inbred (Hartley strain) guinea pigs (GP) were inoculated intragastrically with pathogenic and nonpathogenic Treponema hyodysenteriae. GP 3 to 16 weeks old received T. hyodysenteriae after a fasting period of 36 to 72 h. Infected GP with pathogenic T. hyodysenteriae developed a diarrheal and/or depressive condition, with mucus but not blood in the feces. Of 88 GP, 40 had gross lesion...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 24 4 شماره
صفحات -
تاریخ انتشار 1986